Antibacterial actifity of kecombrang (Etlingera elatior) leaf ethanol extract to against Klebsiella pneumoniae

Eka Puspitasari; Chalies Diah Pratiwi; Dede Rival Novian; Dora Dayu Rahma Turista

doi:10.33503/ebio.v7i01.1663

Eka Puspitasari Department of Medical Analysis, STIKES Hutama Abdi Husada, Tulungagung, Indonesia
Chalies Diah Pratiwi Department of Medical Analysis, STIKES Hutama Abdi Husada, Tulungagung, Indonesia
Dede Rival Novian Department of Veterinary Medicine, Nusa Cendana University, Kupang, Indonesia
Dora Dayu Rahma Turista Department of Biology Education, Mulawarman University, Samarinda, Indonesia

DOI: https://doi.org/10.33503/ebio.v7i01.1663

Keywords: Antibacterial, kecombrang, Klebsiella pneumoniae

Abstract

Infectious diseases caused by bacteria in generals can be cured using antibacterial. The use of antibacterials tends to cause local hypersensitivity either on the skin or mucous membranes so that their use is starting to be reduced. Therefore, the development of natural antibacterials made from plants to reduce resistance is very necessary. kecombrang (Etlingera elatior) is one of the plants that has been shown to have activity in inhibiting bacterial growth. This research is an experimental study that aims to determine the effectiveness of the ethanol extract of the leaves of kecombrang in inhibiting the growth of Klebsiella pneumoniae bacteria in vitro. The method used is the well method with a completely randomized design. The sample came from the Klebsiella pneumoniae bacteria culture. Kecombrang leaf ethanol extract was made in concentrations of 20%, 40%, 60%, 80% and 100%. The extract was tested on Klebsiella pneumoniae bacteria and observed for the formation of inhibition zones. Inhibition the results of the zone measuremens were then analyzed descriptively. The results showed kecombrang leaf ethanol extract 20%, 40%, 60%, 80%, and 100% produce inhibition zone diameter 1.9 mm; 2.6 mm; 7.7 mm; 12.1 mm; and 14.7 mm, 0.4 mm for the negative control and positive control 22.0 mm. Concentration 100% has better antibacterial ability due to higher compound content. The test results show the class of secondary metabolites kecombrang leaf ethanol extract contains flavonoids, tannin, and saponin. The conclusion of this study that the ethanol extract of kecombrang leaves has potential to inhibit the growth of Klebsiella pneumoniae bacteria.

References

Andries, J. R., Gunawan, P. N., & Supit, A. (2014). Uji Efek Anti Bakteri Ekstrak Bunga Cengkeh terhadap Bakteri Streptococcus mutans secara In Vitro. Jurnal Ilmiah Kedokteran Gigi, 2(2), 57–63. https://doi.org/10.35790/eg.2.2.2014.5763

Damanik, D. D. P., Surbakti, N., & Hasibuan, R. (2014). Ekstraksi Katekin dari Daun Gambir (Uncaria gambir roxb) dengan Metode Maserasi. Jurnal Teknik Kimia USU, 3(2), 10–14. https://doi.org/10.32734/jtk.v3i2.1606

Dewi, N., Tarini, N. M. A., & Fatmawati, N. N. D. (2019). Deteksi Gen fimH pada Isolat Klinis Klebsiella pneumoniae di RSUP Sanglah Denpasar. E-Jurnal Medika, 8(4), 1–6. https://ojs.unud.ac.id/index.php/eum/article/view/50051

Elfidasari, D., Noriko, N., Mirasaraswati, A., Feroza, A., & Canadianti, S. F. (2013). Deteksi Bakteri Klebsiella pneumonia pada Beberapa Jenis Rokok Konsumsi Masyarakat. Jurnal Al-Azhar Indonesia Seri Sains Dan Teknologi, 2(1), 41–46. https://doi.org/10.36722/sst.v2i1.97

Fredella, D. M., & Rahman, A. O. (2022). Perbadingan Daya Hambat Minyak Atsiri Green Tea terhadap Bakteri Staphylococcus aureus. Journal of Medical Studies, 2(1), 68–75. https://online-journal.unja.ac.id/joms/article/view/18094

Kurama, G. M., Maarisit, W., Karundeng, E. Z., & Potalangi, N. O. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol daun Benalu Langsat (Dendropthoe sp) terhadap Bakteri Klebsiella pneumoniae. Jurnal Biofarmasetikal Tropis, 3(2), 27–33. https://doi.org/10.55724/j.biofat.trop.v3i2.281

Kusuma, G. A., Longdong, S. N. J., & Tumbol, R. A. (2014). Uji Daya Hambat dari Ekstrak Tanaman Pacar Air (Impatiens balsamina L) terhadap Pertumbuhan Bakteri Aeromonas hydrophila. Jurnal Ilmiah Agrobisnis Perikanan, 2(2), 40–47. https://media.neliti.com/media/publications/44970-ID-uji-daya-hambat-dari-ekstrak-tanaman-pacar-air-impatiens-balsamica-l-terhadap-pe.pdf

Kusumawati, E. (2016). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kecombrang (Etlingera elatior (Jack) R.M. Smith) terhadap Bakteri Bacillus cereus dan Escherichia coli Menggunakan Metode Difusi Sumur. Jurnal Sains Dan Terapan Politeknik Hasnur, 4(1), 26–34. http://ejournal.polihasnur.ac.id/index.php/phssains/article/view/238/223

Kusumawati, E., Supriningrum, R., & Rozadi, R. (2017). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kecombrang Etlingera elatior (Jack) R.M.Sm terhadap Salmonella typhi. Jurnal Ilmiah Manuntung, 1(1), 1–7. https://doi.org/10.51352/jim.v1i1.4

Makmum, A., Surdam, Z., & Gunawan, A. M. (2020). Uji Efektivitas Ekstrak Jintan Hitam (Nigella sativa) terhadap Pertumbuhan Bakteri Staphylococcus aureus Pada Medium MHA (Mueller Hinton Agar). Window of Health : Jurnal Kesehatan, 3(1), 1–9.

Manuaba, I. A. P., Iswari, I. S., & Pinatih, K. J. (2021). Prevalensi Bakteri Escherichia coli dan Klebsiella pneumoniae Penghasil Extended Spectrum Beta Lactamase (ESBL) yang Diisolasi dari Pasien Pneumonia di RSUP Sanglah Periode Tahun 2019-2020. Jurnal Medika Udayan, 10(12), 51–57. https://doi.org/10.24843.MU.2021.V10.i12.P10

Mufti, N., Bahar, E., & Arisanti, D. (2017). Uji Daya Hambat Ekstrak Daun Sawo terhadap Bakteri Escherichia coli secara In Vitro. Jurnal Kesehatan Andalas, 6(2), 289–294. https://doi.org/10.25077/jka.v6i2.693

Narlis, Nasrul, E., & Efrida. (2019). Pola Kuman dan Uji Kepekaan pada Pasien Community Acquired Pneumonia di RSUP Dr. M. Djamil Padang Tahun 2016. Jurnal Kesehatan Andalas, 8(3), 553–557. https://doi.org/10.25077/jka.v8i3.1041

Ngazizah, F. N., Ekowati, N., & Septiana, A. T. (2016). Potensi Daun Trembilungan (Begonia hirtella Link) sebagai Antibakteri dan Antifungi. Jurnal Biosfera, 33(3), 126–133. https://doi.org/10.20884/1.mib.2016.33.3.309

Nurhayati, L. S., Yahdiyani, N., & Hadayatulloh, A. (2020). Perbandingan Pengujian Antivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41–46. https://doi.org/10.24198/jthp.v1i2.27537

Pangestu, I. E., Sumardianto, & Ulfah, A. (2017). Skrinning Senyawa Fitokimia Rumput Laut Sargassum sp. dan Aktivitasnya sebagai Antibakteri terhadap Staphylococcus aureus dan Eschericia coli. Indonesian Journal of Fisheries Science and Thecnologhy, 12(2), 98–102. https://doi.org/10.14710/ijfst.12.2.98-102

Pratama, H. Y., Ernawati, E., & Mahmud, N. R. A. (2018). Uji Antibakteri Ekstrak Kulit Buah Pisang Kepok (Musa paradisiaca x balbisiana) Mentah terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Ilmiah Ilmu Pengetahuan Alam, 7(2), 147–152. https://doi.org/10.35580/sainsmat7273672018

Qurrota, A., Risandiansyah, R., & Fadli, Z. (2020). Efek Kombinasi Alkaloid Ekstrak Phyllanthus niruri L. dengan Amoxicillin atau Chloramphenicol terhadap Daya Hambat Staphylococcus aureus. Jurnal Bio Komplementer Medicine, 7(2), 1–8. https://riset.unisma.ac.id/index.php/jbm/article/view/8939

Ratnah, S., Salasa, A. M., & Ibrahim, I. (2018). Uji Potensi Antimikroba Hasil Fraksinasi Ekstrak Daun Kecombrang (Etlingera elatior) terhadap Candida albicans Penyebab Keputihan pada Ibu Hamil. Media Farmasi, 15(2), 45–50. https://doi.org/10.32382/mf.v14i2.595

Rusli, D. (2017). Formulasi Krim Clindamycin sebagai Anti Jerawat dan Uji Efektivitas terhadap Bakteri Propionibacterium acne. Jurnal Penelitian Sains, 19(2), 82–85. https://doi.org/10.26554/jps.v19i2.478

Sudarwati, D. (2016). Uji Aktivitas Senyawa Antibakteri pada Ekstrak Daun Kelor dan Bunga Rosella. Indonesian Journal of Chemical Science, 5(1), 11–14. http://journal.unnes.ac.id/sju/index/php/ijcs

ARTICLE TEMPLATE

ACCREDITATION CERTIFICATE

COLLABORATION

TOOLS